Fig. 1. Expression of Netrin-1 and its receptors, Neogenin and UNC5H1 in mouse mammary epithelial cells overexpressing Cripto-1. (A) Western blot analysis of expression of Netrin-1, Neogenin and UNC5H1 in the wild type (WT) and Cripto-1 (Cr-1)-overexpressing EpH4 and HC-11 cells. Detection of actin in bottom panel acts as a loading control. (B) Photomicrographs (40x magnification) of immunofluorescence show expression of Netrin-1 and Neogenin in HC-11 cells. Nuclei are stained blue by DAPI. (C) Representative photomicrographs (20x magnification) of histological sections from MMTV-CR-1 transgenic mice mammary tumors containing anaplastic mesenchyme-like tumor cells and stained by immunohistochemistry show low expression of Netrin-1 relative to Neogenin. Sections were counterstained with hematoxylin.