Fig. 5. GST-tau and GST--synuclein each interact with protein complexes containing kinesin-1, whereas only GST--synuclein interacts with protein complexes containing dynein. Western blots of rat brain homogenate incubated with GST, GST-tau or GST--synuclein bound to glutathione-Sepharose beads. Bound proteins were analysed on western blots with antibodies against kinesin-1 (A, B) or dynein (C,D). Panels A and B show an interaction of GST-tau and GST--synuclein with kinesin-1 in brain homogenate, respectively. There is no detectable interaction between GST-tau and dynein (C), whereas GST--synuclein interacts with dynein (D). No interactions were detected in brain homogenate incubated with purified GST alone.