Fig. 6. (A,B) Silver-stained gels and corresponding western blots of immunoprecipitates. (A) Silver-stained gels of immunoprecipitation experiments performed from pooled sucrose gradient fractions. The precipitating antibodies (Ab) and the fractions (F) used for each experiment are noted at the top of the lanes. The panel on the left is an 8% polyacrylamide gel whereas the panel on the right is a 12% polyacrylamide gel. The precipitating immunoglobulin heavy and light chains are labeled HC and LC, respectively. (B) Corresponding western blots using our anti-calmodulin and anti-PF6-IP4 antibodies. All six polypeptides in the PF6 complex precipitate from the 12S fraction. Only subsets of these polypeptides are precipitated from the 6S fraction.