Fig. 5. Bves response to persistent low Ca²⁺. EMCs were passaged in low Ca²⁺ medium (A-C). Subconfluent cells, labeled with Bves and actin-staining phalloidin (A) show that Bves is found at the cell periphery only where two cells appose (arrows). Confluent EMCs are labeled with Bves and ZO-1 (B) or E-cadherin (C). Note that E-cadherin is lost from the membrane in low Ca²⁺, but shares the same membrane pattern as Bves in cells cultured in complete medium (C, inserts).