Fig. 7. The C terminus of Bves interacts with the ZO-1 complex. (A) Diagram of the GST constructs used for a GST pulldown assay. (B) Western blot analysis indicates an interaction between the multimolecular protein complex containing ZO-1 and GST-Bves C terminus recombinant protein following GST pulldown. An antibody to ZO-1 detects an 225 kDa band in 10 µg and 20 µg of MDCK cell lysate. The presence of a similar band is visualized in the protein fraction eluted from beads bound with GST-C-term Bves. (C) Occludin is not detected in the protein fraction bound to any glutathione beads, but the protein is clearly expressed in 20 µg of MDCK cell lysate.