Fig. 10. Immunostaining of starfish eggs with a monoclonal antibody raised against tethering factor. (A) A ubiquitous, 1 µm diameter, extranuclear compartment is labeled by a monoclonal antibody raised against the purified tethering complex. This pattern is highly consistent with yolk granule labeling. (B) Higher magnification reveals that the intracellular compartment staining is peripheral (arrowheads). This is consistent with labeling of a peripheral protein. (C) At a low-gain setting, intense staining of the plasma membrane is emphasized in this confocal micrograph. Bar, 10 µm (A,C); 1 µm (B).