JCS -- McNeil and McNeil 118 (20): 4701 Figure IG4

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Fig. 4. Restoration of tethering by cytosol. (A) After washing in KI, granules are not capable of the tethering response. (B) Addition of cytosol (100 µg) restores tethering. (C) Ca²⁺ addition (300 µM) to the non-tethered population of granules produces small fusion products only. (D) Granules tethered by prior cytosol addition, by contrast, fuse upon Ca²⁺ addition to form large patch vesicle products. Bar, 10 µm.