Fig. 6. Lack of tethering activity in a crude yolk granule lysate. Yolk granules were incubated with IM buffer only (IM), with equal amount (100 µg) anion-exchange-purified tethering factor (HQ) or with a crude granule lysate (LYS) and tethering activity measured. The buffer only and lysate samples did not differ significantly from one another, whereas both were significantly different from the tethering factor sample (P<0.005). Error bars indicate s.d. (n=6).