Fig. 6. Compensatory expression of SgIII and CgA for CPE in the Cpe^fat mouse. (A) Pituitary homogenates from wild-type mice (WT, lane 1) and the Cpe^fat mouse (fat/fat, lane 2) were analyzed by immunoblotting and probed with antibodies to CPE, SgIII, CgA and -tubulin. The blots were visualized using the ECL kit. (B) The relative levels of CPE, SgIII, CgA and -tubulin were determined by quantitative radio-immunoblotting with pituitary gland homogenates from five WT mice and five Cpe^fat mice. The blots were reacted with polyclonal or monoclonal antibody to CPE, SgIII, CgA and -tubulin (see upper panel) and were probed with ¹²⁵I-labeled secondary antibody. The signals were measured using a BAS2000 and were normalized by the signal obtained from an -tubulin blot as an internal control. The levels of CPE, SgIII, CgA and -tubulin in the Cpe^fat pituitary were expressed by assuming those in the WT pituitary as 100%.