Fig. 1. CCH alters actin organization in lacrimal acini. Confocal fluorescence micrographs of control acini (CON) and acini exposed to 100 µM CCH for 5 minutes (CCH 5) or 15 minutes (CCH 15) and fixed and processed as described in Materials and methods to label actin filaments. The top right panel shows a schematic outline of the actin filaments in CON, with thick lines representing apical actin filaments and thinner lines representing basolateral actin filaments. *, Lumenal regions; arrows, actin-coated structures; arrowheads, increased basolateral actin; bar, 5 µm.