Fig. 4. Cytochalasin D induces the re-distribution of endosomes in the perinuclear region of MNT-1 cells. (A-F) MNT-1 cells that had been treated (D,E,F) or not (A,B,C) with cytochalasin D were co-labelled with anti-EEA1 antibody (A,D), and phalloidin (B,E), and then analysed using confocal microscopy. (A,B) and (D,E) show the same confocal sections throughout the nucleus. (C,F) Overlay of (C) A,B and (F) D,E. Arrows show co-distribution of EEA1-labelled compartments with actin filaments. Bar, 2.8 µm.