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Fig. 5. Expression of EGFP-Myo1b perturbs the cellular distribution of Pmel17. (A,B) EGFP-Myo1b-expressing (A) or EGFP-expressing (B) MNT-1 cells labelled with anti-Pmel17 antibody (HMB50) and analysed by confocal microscopy. Overlay of the optical section through the nucleus shows the distribution of Pmel17 and EGFP-Myo1b (A). Overlay of the optical sections throughout the nucleus showing the distribution of Pmel17 and EGFP (B). (C,D) Pmel17-expressing HeLa cells that had been transiently transfected with cDNA encoding (C) EGFP-Myo1b and (D) EGFP, were labelled with anti-Pmel17 antibody (HMB50) and analysed by confocal microscopy. Overlay of the optical sections through the nucleus show the distribution of (C) Pmel17 and EGFP-Myo1b, and (D) Pmel17 and EGFP. Notice the re-distribution of Pmel17 (arrows) towards the nucleus in EGFP-Myo1b-expressing cells. Bars, 1.9 µm.