Fig. 10. Effects of a positive curvature agent, LPC, on CV-CV fusion. Ca²⁺ activity curves of untreated CVs and CVs treated with (A) 10 µM LPC, or 2 mM mßcd, or treated first with 2 mM mßcd followed by treatment with 10 µM LPC (n=3) as indicated. Vertical dashed lines indicate the EC50 of each curve. (B) Samples paralled to A first treated with 10 µM LPC and subsequently with 1 mM hpßcd (n=3). Following sequential treatments with 2 mM mßcd and 10 µM LPC, samples were treated with 1 mM hpßcd, or 1 mM hpßcd-cholesterol (n=3). Blue line indicates the 2 mM mßcd-treated sample as in A.