Fig. 6. Addition of exogenous cholesterol to cholesterol-depleted CVs. Homotypic CV-CV fusion assays of CVs treated with 0 mM or 2 mM mßcd, and recovered by delivering cholesterol using (A) 2 mM mßcd as a vehicle (n=3), saturated cholesterol solutions (n=4), and (B) with 2 mM hpßcd as a vehicle as indicated(n=4). Vertical dashed lines in A and B indicate the EC50 of each curve. (C) Kinetics of CV-CV fusion triggered with 67±14 µM [Ca²⁺]_free (n=4), after treatments as in A and B. (D) Total CV cholesterol assayed before and after 2 mM mßcd treatment, and with subsequent recovery with the indicated delivery methods. *Significant difference from control and all other conditions (P<0.005); **,***significant differences from control and all other conditions (P<0.05).