JCS -- Churchward et al. 118 (20): 4833 Figure IG9

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 9. The effects of sterol binding antibiotics on CV-CV fusion. (A) Ca²⁺ activity curves of CVs treated with concentrations of filipin III as indicated (n=4). (B) Ca²⁺ activity curves of CVs treated with concentrations of pimaricin as indicated. (C) Ca²⁺ activity curves of CVs treated with concentrations of amphotericin B as indicated. Vertical dashed lines in A-C indicate the EC50 of each curve. (D) Fusion kinetics of CVs treated as in A and B, or CVs treated with 50 µM or 100 µM pimaricin in response to 104±10 µM [Ca²⁺]_free (n=2-3).