Fig. 9. In vivo association of paxillin and Crk in NMuMG cells. Lysates were prepared from either untreated (–) or TGF-ß1-treated (+) NMuMG cells for coimmunoprecipitation experiments. (A) Detergent soluble lysates (DSL) were run alongside control (IgG) or Crk immunoprecipitates on standard SDS-PAGE. Immunoblots were performed using specific antibodies to the indicated proteins and as indicated to their phosphorylated isoforms. (B) DSL and p120RasGAP immunoprecipitates were loaded and run on standard SDS-PAGE. Immunoblots were performed using specific antibodies to the indicated proteins. (C) Detergent-soluble lysates (DSL) were run alongside control (IgG) and paxillin (clone 165 antibody) immunoprecipitates, prepared from NMuMG cells treated with TGF-ß1 for 48 hours, on standard SDS-PAGE. Immunoblots were performed using specific antibodies to the indicated proteins.