Fig. 5. K-fibres form in the absence of Cenp-F. (A) Control and Cenp-F RNAi cells were extracted to preserve K-fibres, then fixed and stained to detect tubulin (green) and the DNA (red). Note that although the spindle appears aberrant in Cenp-F-repressed cells, K-fibres are apparent. (B) Images of nocodazole-arrested cells stained to detect Cenp-F (green) and Hec1 (red) showing that Hec1 localizes to Cenp-F-depleted kinetochores. Scale bars represent 5 µm. (C) Graph plotting inter-kinetochore distances, in µm, using Cenp-A as a marker. Measurements are made using control cells in either prometaphase or metaphase, and Cenp-F deficient cells, horizontal lines represent the means.