Fig. 1. PANC-1 and BxPC-3 cells were serum-starved for 24 hours and treated with solvent (–TGFß) or 10 ng/ml TGFß₁ (+TGFß₁) for 2 days. (A) The left panel shows phase contrast pictures. (B) The right panel shows immunofluorescence staining of E-cadherin. Bars, 20 µm. (C) Western blot analyses of E-cadherin, - and ß-catenin in total lysates of PANC-1 and BxPC-3 cells treated with 10 ng/ml TGFß₁ for the indicated periods of time. Equal loading was demonstrated by staining of ß-actin. Molecular mass standards are given in M_r x10³. Representative blots out of four independent experiments are shown.