Fig. 6. (A) Immunoprecipitation of E-cadherin, - or ß-catenin was performed from 500 µg of PANC-1 RIPA lysate and PTEN was detected by immunoblotting. Equal amounts of immunoprecipitated proteins were documented by restaining the blots with the appropriated antibody. (B) Beta-catenin was precipitated from 2 mg of BxPC-3 or PANC-1 lysates treated with 10 ng/ml TGFß₁ or solvent. Co-precipitated PTEN was detected by western blotting. The blots were restained with anti-ß-catenin antibody to demonstrate equal amounts of protein. (C) E-cadherin was precipitated from 0.5 mg of total lysate from PANC-1 cells transiently transfected with PTEN, PTENC or vector alone (mock). Co-precipitated - and ß-catenin was determined by western blotting. Restaining of the blot for E-cadherin confirmed equal amounts of precipitated proteins. In all experiments representative blots out of three independent studies are shown.