Fig. 1. (A) Changes in nuclear volume induced by HDACi (TSA and NaBt) determined in two human cell types (A549 and HT29). Average nuclear volume ± s.e.m. was calculated for each treatment and was related to the control values (100%). Asterisks indicate results significantly different from the control; P<0.05. (B) Chromatin decondensation of classical satellites of chromosome 9 in control and TSA-treated A549 cells. Bar, 2 µm. (C) The induction of apoptosis by HDACi was confirmed in A549 and HT29 cells by western blots that show the cleavage of PARP (120 kDa) into 80 kDa fragments and cleavage of lamin B (65 kDa) into 45 kDa fragments.