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Fig. 2. (A) Flow cytometric detection of the cell-cycle profiles in TSA- and NaBt-treated A549 and HT29 cells. Modfit software analysis shows G1 (red), S (hatched) and G2 (green) stages of the cell cycle. NaBt induced accumulation of cells in G1 while TSA increased the number of cells in G2. (B) Western blot detection of dimethylated H3(K9) (17 kDa), acetylated H3(K9) (17 kDa) and dimethylated H3(K4) (17 kDa) in A549 cells treated with TSA and NaBt. The western blot data were related to the selected band (arrow) of total nuclear protein levels. The figures show an illustration of representative blots.