Fig. 7. Confocal sections of cell nuclei at different z-positions (S1, S2 and S3) and maximum images (bottom) are shown for nuclei of A549 cells. The H3(K4) dimethylation pattern (green signals) was determined by immunocytochemistry, and centromeric regions (classical satellites of chromosome 9; red signals) were stained using the FISH technique. In both control and TSA-treated cells the centromeres were located in regions lacking H3(K4) dimethylation. Bar, 1 µm.