Fig. 7. NOSTRIN-dependent translocation of eNOS requires an intact actin cytoskeleton. CHO-eNOS cells infected with SFV encoding NOSTRIN for 8 hours were treated with cytochalasin D within the last 2 hours of infection (D-F) or mock-treated (A-C) as control. After fixation, cells were stained with anti-NOSTRIN (B,E) and anti-eNOS (A,D). NOSTRIN and eNOS co-localize in intracellular vesicle-like structures of untreated cells (A-C), whereas they accumulate in large patches in the periphery of cytochalasin-treated cells (arrows) (D-F). Bars, 10 µm.