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Fig. 1. Absence of wild-type granulosa cell contamination in ovaries constructed from wild-type oocytes and Cx43-deficient somatic cells. 43+/+oo/43+/+gc (A,C,E) and 43+/+oo/43–/–gc (B,D,F) ovary sections were immunostained for Cx43. Punctate immunoreactivity characteristic of gap junctions is evident between the granulosa cells of 43+/+oo/43+/+gc chimeric ovaries (C,E) but not 43+/+oo/43–/–gc chimeric ovaries (D,F). E and F are magnifications of C and D, respectively. Bar, 50 µm (C,D); 10 µm (E,F).