Fig. 1. CNP antagonizes FGF2-mediated inhibition of RCS proliferation. (A,B) Cells were treated with FGF2 and either CNP or pCPT-cGMP for 72 hours and counted. Data represent the average from four wells with the indicated standard deviation. (B) Cells not treated with FGF2 were given an artificial value of 0.08 on logarithmic x-axis. (C) Cells were treated with FGF2 and either CNP (Experiment 1) or pCPT-cGMP (Experiment 2) for 24 hours and analyzed for DNA content by flow-cytometry. Note the FGF2-mediated accumulation of cells in the G1 phase of cell cycle that was partially reversed by CNP or pCPT-cGMP.