Fig. 9. FGF2 and CNP effects on extracellular matrix homeostasis. (A,C) Cells were treated with FGF2 and CNP for 48 hours in the presence of [³⁵S]sulfate (A) or [³H]proline (C) and the amount of incorporated radioactivity in the cell layer was determined by liquid scintillation. Note the potent regulation of [³⁵S]sulfate incorporation by both FGF2 and CNP in contrast to [³H]proline incorporation that was only mildly enhanced by CNP. (B,D) Cells were pulsed with [³⁵S]sulfate (B) or [³H]proline (D) for 12 hours prior to 48 or 72 hours of treatment with FGF2 and CNP. The remaining incorporated radioactivity was determined by liquid scintillation. Note that the FGF2-mediated decrease of incorporated [³H]proline required 72 hours of treatment. The data represent the average from six wells with the indicated standard deviations. (E) Cells were treated with FGF2 and CNP for 48 hours, harvested by either scraping or trypsinization and both the wet and dry mass of the pellets was determined. The data represent the average from eight wells with the indicated standard deviations. Statistically significant differences are highlighted (ANOVA; ^*P<0.05, ^**P<0.01).