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Fig. 2. Expression and localization of paracellin-1. (A) Constitutive expression of paracellin-1 in MDCK-II and LLC-PK1 cells. Paracellin-1 migrates as a 27 kDa band (*). (B) Confocal microscopy reveals the colocalization of paracellin-1 with occludin at the tight junction. Red, occludin staining; green, paracellin-1 staining; yellow to orange, colocalization of occludin and paracellin-1 on the merged panel. Bar, 10 µm.