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Fig. 5. Generation of a murine fibroblast line overexpressing human CD44s. MDA-MB-468 cells, MDA-MB-231 cells and NIH-3T3 cells transfected with pSR{alpha} vector alone and NIH-3T3 cells expressing human CD44s were lysed and subjected to western blotting with anti-human CD44 monoclonal antibody E1/2, monoclonal antibody IM7, which recognises both human and murine CD44, or anti-murine CD44 monoclonal antibody KM201. Positions of molecular size markers are indicated in kDa.