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Fig. 8. Reversibility of IFN{gamma} effects on claudin-1 and occludin in T84 membranes fractions. T84 monolayers were treated for 48 hours with 10ng/ml IFN{gamma} followed by removal of the cytokine for a further period of 96 hours as indicated and then extracted as described in Materials and Methods. Triton-soluble (S) and Triton-insoluble (I) fractions were immunoblotted with antibodies to claudin-1 and occludin. Images are representative of three separate experiments.