Fig. 4. Lack of colocalisation between Dvl2 puncta and endocytic markers. COS-7 cells expressing HA-Dvl2, were fixed and double-stained with antibodies against HA (green), and various endocytic markers (red) as indicated. (A-C) Endogenous endocytic and Golgi proteins; (D-F) exogenous endocytic proteins. (G,H) Overexpression of dominant-negative mutants that block endocytosis, which neither affected the abundance nor the distribution of the Dvl2 puncta. Bars, 15 µm.