Fig. 8. Morphology of GFP-Dvl2 puncta at high magnification. (A,B) Single confocal sections of COS-7 cells expressing GFP-Dvl2; (C) GFP-Dvl2 puncta (green in merged image) were also stained with anti-Dvl2 antibody (red in merge); high magnification view in (B) corresponds to boxed section in (A). Notice the multi-centred `puncta' in (B). (D-F) Single confocal sections through individual GFP-Dvl2 puncta (bottom left) and orthogonal reconstructions along the two coloured axes as indicated (above and to the right, respectively) from image stacks. (D,E) deconvoluted images; (F) original image corresponding to (E). All cells were fixed before imaging.