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Fig. 5. Domain dependence of Dvl-2 localisation in transient ectopic expression systems. (A-F) Immunolocalisation of HA-Dvl-2-ER proteins transiently transfected into MDCK cells. Cells were fixed 1 hour after re-feeding with medium containing 1 µM ß-estradiol or ethanol vehicle. (A) {Delta}DIX HA-Dvl-2-ER vehicle only. (B) {Delta}DIX HA-Dvl-2-ER treated with 1 µM estradiol. (C) {Delta}PDZ HA-Dvl-2-ER vehicle only. (D) {Delta}PDZ HA-Dvl-2-ER treated with 1 µM estradiol. (E) K446M HA-Dvl-2-ER vehicle only. (F) K446M HA-Dvl-2-ER treated with 1 µM estradiol. (G-J) Staining pattern of `actin mutant' and `vesicle mutant' Dvl-2 transfected into HEK293 cells. Dvl-2 localisation is in green and nuclei were counterstained with ToPro III (blue). (G) Wild-type HA-Dvl-2-EGFP. (H) HA-Dvl-2-EGFP K58A. (I,J) HA-Dvl-2-EGFP K68A,E69A. Bar, 8 µm.