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Fig. 6. Domain dependence of Dvl-2-ER localisation in single-copy cell lines. All panels were stained with anti-HA antibody. (A) Parental HEK293 cells treated with vehicle only for 1 hour. (B) Parental HEK293 cells treated with 1 µM ß-estradiol for 1 hour. (C) Wild-type HA-Dvl-2-ER line vehicle-only control. (D) Wild-type HA-Dvl-2-ER line treated with 1 µM ß-estradiol for 1 hour. Inset is a close-up of HA-Dvl-2-ER localisation at MTOC (arrow) and at cell membranes (arrowheads). (E) {Delta}DIX HA-Dvl-2-ER line vehicle-only control. (F) {Delta}DIX HA-Dvl-2-ER line treated with ß-estradiol. Inset is a close-up of HA-Dvl-2-ER localisation at MTOC (arrow) and at cell membranes (arrowheads). (G) {Delta}PDZ HA-Dvl-2-ER line vehicle-only control. (H) {Delta}PDZ HA-Dvl-2-ER line treated with ß-estradiol. Note identical staining to control cells. (I) K446M HA-Dvl-2-ER line vehicle-only control. (J) K446M HA-Dvl-2-ER line treated with ß-estradiol. Inset is a close-up of HA-Dvl-2-ER localisation at MTOC (arrow) and at cell membranes (arrowheads). Bar, 40 µm.