Fig. 7. Time-lapse analysis of HA-Dvl-2-EGFP puncta. (A) Still frames from Movie 1B in supplementary material, illustrating lack of coordinated or directional movement in fluorescent bodies in a cell ectopically expressing HA-Dvl-2-EGFP at high levels. Frame numbers are indicated. Four bodies are false-coloured in the first frame (213) so that their fate may be followed. Note the yellow and green bodies fuse in frame 230 and the orange and red bodies fuse via a `dumb-bell' intermediate in frame 263. (B) Still frames from Movie 1C in supplementary material, illustrating lack of coordinated or directional movement in fluorescent bodies in a cell expressing lower levels of HA-Dvl-2-EGFP. Frame numbers are indicated. Two bodies are false coloured in the first frame (256) so that their fate may be followed. Note the red body appeared to split into two between frames 293 and 308, the yellow body disappeared by frame 314 and one of the two bodies resulting from the split of the red body disappeared by frame 328. Bars, 12 µm.