Fig. 7. LPA stimulation leads to the colocalization of ß-arrestin-2-GFP with clathrin AP2 adaptors. LPA₁/HeLa cells were transiently-transfected with plasmids encoding ß-arrestin-2-GFP and incubated with 10 µM LPA for either 0 minute or 2 minutes prior to fixation. Endogenous clathrin AP2 was localized in permeabilized cells using mouse anti-AP2 antibodies. The inset shows a high magnification image of the boxed region and the arrows indicate structures where ß-arrestin-2-GFP colocalized with AP2. Bar, 10 µm.