Fig. 2. Cells were de-roofed and an AFM image recorded (A, deflection and B, height). Parts of the ventral plasma membrane were preserved during the de-roofing procedure (B, arrows). (C) The localization of paxillin-YFP within an area corresponding to the dashed box in (B) was determined by fluorescence microscopy. (D) Overlaying the AFM height image with the paxillin fluorescence image highlights paxillin-containing FAs. (E) Several paxillin-positive protein complexes (arrows) were subsequently imaged at higher resolution by AFM (E, height and F, deflection signal; scan area corresponding to the dashed box in D). Filamentous structures arranged at slight angles to each other can be distinguished in some of the FAs. The full range of the height scale corresponds to heights of 600 nm (B,D) and 350 nm (E). Bar, 10 µm (A-D) and 1 µm (E,F).