Fig. 2. Time course of I_HERG decline induced by 10 µM ceramide. (A) Typical recording obtained from a cell in control solution and then after 1 hour exposure to 10 µM ceramide. Tail currents at –60 mV were recorded following depolarisation to +40 mV for 2 seconds from the holding potential of –80 mV. The protocol was repeated every 12 seconds. (B) Plot of change in I_HERG density from baseline against time. A baseline was established during 5-10 minutes of perfusion with control solution and then solutions were changed to 10 µM ceramide () or control (). Protocol as in A with I_HERG taken as the peak tail current. The number of cells tested are given in parenthesis (^*P<0.05, ^**P<0.001).