Fig. 2. Anaphase microtubule reorganization fails in tum mutant cells. (A-D) Tum localisation in wild-type dividing cells. DNA (A and blue in D), Tum (B and red in D) and microtubules (C and green in D). Prometaphase cells show diffuse Tum staining (asterisks in B). An anaphase cell (arrowhead, B) has Tum localised at the cortex and in microtubule bundles. A telophase cell (arrow, B) has partially completed cytokinesis and has a ring of Tum. Residual midbodies mark the positions of previous divisions (arrows, D). This is a merged Z-stack representing a total 4-µm-thick slice. (E-H) Tum localisation in dividing cells of the tum^DH15 mutant at stage 12. Tum alone (F) and merged (H, in red) with microtubules in green and DNA in blue. Tum is not detectable in embryos at this time (F). Metaphase cells (arrows, G) appear normal, whereas anaphase and telophase cells (asterisks, G) have a variety of aberrant microtubule bundles. This is a merged Z-stack representing a total 1-µm-thick slice. Bar, 5 µm.