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Fig. 3. Induced cells build a flagellum, however daughter kinetoplast position is impaired. Phase-contrast images of control cells (A,B) and TbPACRG AB RNAi-induced cells at 72 hours (C-F) with nuclear and kinetoplast DNA overlaid in blue. (A) A non-induced cell with one kinetoplast and one nucleus (1K1N). (B) A non-induced cell with duplicated nucleus and kinetoplast (2K2N cell) showing correct positioning of both kinetoplasts. (C) A TbPACRG AB RNAi-induced cell with correct positioning of the kinetoplast. (D) A TbPACRG AB RNAi-induced cell undergoing division where the distance between the daughter kinetoplast (arrow) and the daughter nucleus is reduced compared to that of the parent cell. Note the formation of the new flagellum (NF). (E) A TbPACRG AB RNAi-induced 1K1N cell where the kinetoplast is mislocalised. (F) A TbPACRG AB RNAi-induced cell undergoing cytokinesis where the daughter kinetoplast (arrow) is mispositioned, anterior to the daughter nucleus. Bar, 5 µm.