Fig. 5. Effect of CYP 2C9-overexpression on matrix metalloprotease (MMP) activity. (A) Human endothelial cells were infected with either CYP 2C9 sense (2C9) or antisense (CTL) adenoviruses in the absence or presence of sulfaphenazole (Sulfa, 30 µmol/l) for 24 hours. MMP activity was determined using a commercially available MMP activity assay. Results are presented as the mean±s.e.m. of data obtained in five to seven independent experiments. Zymography using the supernatant of the cells (B) infected with CYP 2C9 sense (2C9) or antisense (CTL) adenoviruses and cultured for 48 hours or (C) treated with 11,12-EET (1 µmol/l) for the indicated times and myelin basic protein (MBP) as a substrate. The bar graph summarizes the results of five independent experiments; ^*P<0.05 vs the level in the control (CTL).