Fig. 8. Effect of hypoxia on CYP 2C expression, migration and tube formation by porcine coronary artery endothelial cells. (A) Representative western blot showing the time-dependent induction of CYP 2C by hypoxia. Porcine liver served as a positive control (pc). (B) Endothelial cells were cultured under normoxic or hypoxic conditions in the absence or presence of 14,15-EEZE (EEZE, 10 µmol/l) or MS-PPOH (MS, 10 µmol/l) for 24 hours and then seeded onto Transwell filters. The number of cells that had migrated through the filters was counted after 20 hours. (C) Endothelial cells, cultured under normoxic or hypoxic conditions, were seeded onto Matrigel and branching points were counted after 14 hours. The bar graphs show the mean±s.e.m. of three to four independent experiments; ^*P<0.05, ^**P<0.01 vs the level in solvent-treated cells (Sol) maintained in normoxia.