Fig. 3. Turnover of EPMH reporters in bloodstream trypanosomes Transgenic bloodstream cell lines expressing either EPMH (panel A) or EPMHgpi (panel B) were pulse radiolabeled with [³⁵S]Met/Cys for 15 minutes and then chased with complete media. At the indicated times polypeptides were immunoprecipitated from cell and media fractions with either anti-EP₉ (2x10⁷ cell equivalents) or anti-VSG (2x10⁵ cell equivalents). Samples were analyzed by SDS-PAGE and phosphorimaging. (A) `M' and `P' indicate the mobility of mature and precursor EPMH polypeptides. (B) As indicated, the EPHMgpi cells were either untreated or treated with FMK024 (20 µM) for 15 minutes prior to radiolabeling, and subsequently for the duration of the pulse/chase periods. Vertical white stripes indicate irrelevant regions of phosphoimages that were digitally removed to simplify presentation. The mobilities of molecular mass markers are indicated in kDa.