JCS -- Schwartz et al. 118 (23): 5499 Figure IG6

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Fig. 6. Immunolocalization of EPMH ${Delta}$ gpi. Bloodstream cells expressing EPMH ${Delta}$ gpi were mock-treated (panels A-L; -) or cultured with FMK024 for 1 hour (panels M-T; +). Cells were fixed/permeabilized, stained with the indicated antibodies, and visualized by epifluorescence microscopy. (A-D,M-P) Stained with anti-EP₉ and anti-BiP antibodies. (E-L,Q-T) Stained with anti-EP₉ and anti-p67 antibodies. All panels: left column, merged DIC/DAPI images; right column, merged three channel fluorescent images (arrowheads indicate lysosomal localization). DAPI staining (blue) reveals nucleus (n) and kinetoplast (k) localization (indicated in A only).