Fig. 4. Expression of FLNa and CEACAM1-L affects cell migration. (A) Cell migration of M2, A7, M2-CC1 and A7-CC1 cells was determined in a Transwell Boyden chamber assay. Mean migrations relative to M2 wild type cells in 3 independent experiments performed in triplicate are shown with their standard deviations. Student's t-test revealed (^*1) P<0.005 compared with M2, and (^*2) P<0.01 compared with A7 cells. (B) Cell invasion of M2, A7, M2-CC1 and A7-CC1 cells was determined using BD Biocoat Matrigel Invasion Chamber assays. Mean invasions relative to M2 wild-type cells in three independent experiments performed in triplicate are shown with their standard deviations. Student's t-test revealed (^*3) P<0.006 compared with M2, and (^*4) P<0.01 compared with A7 cells.