Fig. 5. Contribution of myosin contractility to junctional actin and thin bundles. Confluent keratinocytes grown in low calcium medium (Low Ca²⁺) or induced to form junctions (Std. Ca²⁺) for 1 hour were incubated in the presence or absence of blebbistatin (Bleb.). Controls were treated with DMSO. Enlargements of the boxed regions in each image are shown on the right. In the absence of cell-cell contacts (Low Ca²⁺) thin bundles are present in control (DMSO-treated) cells, but not in blebbistatin-treated cells (Bleb.) After cell-cell adhesion, junctional actin is formed in the absence of thin bundles (Bleb. Std. Ca²⁺). Asterisks indicate the absence of thin bundles in blebbistatin-treated keratinocytes; arrow indicates junctional actin and arrowheads, wavy junctions. Images are representative of at least three independent experiments. Bar, 50 µm.