Fig. 6. Following cell-cell junction formation, phosphorylated myosin light chain (P-MLC) localizes at thin bundles but not junctional actin. Keratinocytes grown in low calcium medium (Low Ca²⁺) were induced to form junctions (Std. Ca²⁺) and stained with phalloidin and anti-P-MLC. Merged confocal images are shown on the right; Enlargements of the boxed regions are shown below each image. After induction of cell-cell contacts, P-MLC is concentrated at peripheral thin bundles, but absent from junctional actin (Std. Ca²⁺). Arrowheads show junctional actin, arrows point to thin bundles and localization of P-MLC. Asterisks show absence of P-MLC at junctional actin. Bar, 32 µm; 8 µm for enlargements.