Fig. 6. Lifespan analysis of nph-1 and nph-4 single and double mutants in C. elegans. The lifespan of nph-1, nph-4 and nph-1;nph-4 double mutants was compared to N2 wild-type controls and the long lived osm-5 cilia mutants. The nph-1(ok500), nph-4(tm925) and nph-1(ok500); nph-4(tm925) double mutant strains had a significant expansion in lifespan compared to the N2 worms. Although the nph-1(ok500) and nph-1(ok500); nph-4(tm925) double mutants did not have as severe of an increase in lifespan as osm-5 mutant worms, single nph-4(tm925) mutants were indistinguishable from osm-5 mutant worms. Similar data were obtained on three independent lifespan experiments (average of 75 total worms in each genetic category) conducted for this analysis.