Fig. 3. Expression of PfGRASP-GFP in transgenic parasites. (A) Immunoblot using GFP-specific antibodies on wild-type (WT) and PfGRASP-GFP expressing parasites (GRASP-GFP). A band of 100 kDa, representing the GFP-fusion protein, is recognized by GFP-specific antibodies in the transgenic, but not in the WT parasite line. In addition, two smaller bands, possibly GFP breakdown products can be detected. (B) Anti-PfGRASP-specific antibodies recognize an 100 kDa PfGRASP-GFP fusion protein in addition to the endogenous PfGRASP protein of 70 kDa in PfGRASP-GFP-expressing parasites.