Fig. 4. Localisation of PfGRASP by confocal and fluorescence microscopy in trophozoites (<24 hours post invasion). (A) Full-length PfGRASP is expressed as a GFP-fusion protein. Using fluorescence of the GFP reporter protein in live cells, PfGRASP-GFP distribution (green) is restricted to two compartments within the parasite (a) (see also supplementary material Movie 1). These compartments are in close proximity to the nucleus (b, blue). Merge with bright-field image (c). (B) Fixed WT parasites were incubated with PfGRASP-specific antibodies. PfGRASP-specific antibodies (a, red) show a similar fluorescence pattern in fixed cells compared with PfGRASP-GFP expressing parasites. However, additional unspecific staining can be detected. Merge with DNA-specific stain (b, blue). Merge with bright-field image (c). Bar, 2 µm.