Fig. 2. Time-dependent effect of CAI on CCE inhibition. Thapsigargin (1 µM) and Ca²⁺ (2 mM) were added to a suspension of HEK-293 cells. (A) CAI (10 µM) was added either 45 seconds (thin solid trace) or 300 seconds (dashed line) before Ca²⁺ and the CCE increase was compared with that in control cells (thick solid trace) and the [Ca²⁺]_i increase in the absence of Ca²⁺ store depletion (basal, not shown). (B) Mean results from a series of experiments similar to those shown in A. Values are the mean±s.e.m.; n=39, 3, 10 and 4 for control, CAI 300 seconds, CAI 45 seconds and basal, respectively.